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#1338422 - 04/15/13 02:06 PM Having a Necropsy/Histopath done the PROPER way!!
Srlb Offline
Glideritis Anonymous

Registered: 10/30/03
Posts: 16757
Loc: St. Johns, Florida
I have received many emails over the years asking the best way to preserve the body of one of our lost sugar gliders for a necropsy. The correct answer is REFRIGERATE the body. DO NOT EVER FREEZE it!!

Recently I was contacted by a sugar glider owner asking me about this as she knows of someone that was told to freeze the bodies. Again DO NOT EVER FREEZE a sugar gliders body.

Get your sugar glider in to your local vet within 1-2 days for a necropsy. They will then preserve the tissues/organs that need to be sent off. One of the best places to ask your veterinarian to send your babies tissues to would be http://www.zooexotic.com/ Zoo Exoctic Pathology. They specialize in exotics, including sugar gliders. Many place and schools that do histopaths on sugar gliders are not as well versed in the field of exotics of this kind. Many schools will do these as an educational learning tool, but when we are trying to find the cause of death on our baby, do we really want someone who is just learning to be the one to try and find the answers?

Now why do I stand so firm on the process of reserving the bodies?

Antioch Diagnostics state:

“How Do We Preserve The Carcass? Ideally carcasses should be kept refrigerated but not frozen. If the carcass is adequately refrigerated soon after death, the necropsy can be diagnostic for as long as a week after death. Freezing, however, can damage tissues and make the histopath more difficult. Even so, we can often still make some diagnoses despite the freezing. If you have already frozen the carcass, keep it frozen for delivery so we can do a special controlled thaw. Depending on the size of the carcass, our controlled thawing process can take several days. “
Can be found http://antechnecropsy.com/

According to the Animal Hospitals –USA:

“If any delay in necropsy is anticipated, the body should be refrigerated immediately but not frozen. Freezing produces significant changes in the tissues and interferes with the microscopic interpretation of tissue samples. Freezing of the cadaver may be necessary in certain circumstances, but it should be avoided whenever possible.”
Can be found here: http://www.animalhospitals-usa.com/birds/bird-diseases/necropsy.html

I went one step further and sent off an email to Zoo Exotic Pathology (http://www.zooexotic.com/) and would like to share them with you now:

Quote:
On Apr 15, 2013, at 9:05 AM, <critterlove@critterlove.com> <critterlove@critterlove.com> wrote:

In order to receive the best results in a histopath performed on a sugar glider, do you recommend the owner to refrigerate or freeze the body prior to having a necropsy done?

Have a great day and smile!
Peggy Brewer
Critter Love
www.critterlove.com
www.hpwdietcenter.com



Quote:
From: Drury Reavill [mailto:DReavill@zooexotic.com]
Sent: Monday, April 15, 2013 12:54 PM
To: critterlove@critterlove.comcritterlove@critterlove.com
Subject: Re: Histopath

Never, ever freeze tissues on which you are planning to do histology! Post mortem examinations need to be done within 1-2 days of death for meaningful results. The GIT of rodents and many marsupials rot quickly because of the type of diet and gut these critters have. The body should be kept cold, NOT FROZEN.

Thank you,
Drury

Drury Reavill, DVM, DABVP, certified in avian practice, DACVP
Zoo/Exotic Pathology Service
Shipping Address: 2825 KOVR Drive
West Sacramento, CA 95605
(916) 725-5100
(800) 457-7981
FAX (916) 725-6155
Dreavill@zooexotic.com

Website: www.zooexotic.com
Follow us on Facebook,www.facebook.com/ZEPathologyService?ref=mf , and Twitter!

Association of Avian veterinarians (AAV) www.aav.org
Association of Exotic Mammal veterinarians (AEMV) www.aemv.org
Association of Reptile and Amphibian veterinarians (ARAV) www.arav.org
Veterinary Information Network www.vin.com


Before posting this over here, I wrote back and asked permission to share the emails, which I received, but they also added the following:

Quote:
Yes, you may. Remember, for histologic evaluation, the tissues should never be frozen, they should be 'fresh'. Some of your information may be coming from people quoting in-surgical evaluation of tumors. Those biopsies are collected during the surgery and frozen to be read immediately, so the surgeon can decide how much more he/she needs to cut. Only really happens in human medicine and is only run on people on the surgeons table.

This might be a reference for you: http://books.google.com/books?id=JdtgE0e...psy&f=false

Thank you,
Drury

Drury Reavill, DVM, DABVP, certified in avian practice, DACVP
Zoo/Exotic Pathology Service
Shipping Address: 2825 KOVR Drive
West Sacramento, CA 95605
(916) 725-5100
(800) 457-7981
FAX (916) 725-6155
Dreavill@zooexotic.com

Website: www.zooexotic.com
Follow us on Facebook,www.facebook.com/ZEPathologyService?ref=mf , and Twitter!

Association of Avian veterinarians (AAV) www.aav.org
Association of Exotic Mammal veterinarians (AEMV) www.aemv.org
Association of Reptile and Amphibian veterinarians (ARAV) www.arav.org
Veterinary Information Network www.vin.com




So for you out there that are being told to freeze your sugar gliders, please know that by doing this you will NOT be receiving the BEST result on your little one. Maybe this is why we are not getting more answers and histopaths are coming back inconclusive, because the tissues and organs are being destroyed through freezing.

If you ever see anyone asking this, please be sure to pass along the PROPER information.
_________________________
Peggy
Critter Love
Critter Love® Diet Center

If you want to know what a person is like, watch how he treats others.

You'll never know what the outcome is if you don't step up and try.


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#1338436 - 04/15/13 03:58 PM Re: Having a Necropsy/Histopath done the PROPER way!! [Re: Srlb]
josefine Offline
Glider Addict

Registered: 08/17/08
Posts: 2713
Loc: Perry, Iowa
And make sure you get w/Val,'Jackie Chans Mom' here on GC, on the correct way to send your sugar glider for a necropsy report from her.
I didn't do it 100% correctly,so they maybe weren't able to give me a complete answer on my Bella.
On the Sweet Spot,Val is 'Something To Believe In' also.
_________________________
Larry & Josefine Vodenik
2014 4 St
Perry,Iowa50220
515/321-6081cell#
j.vodenik@hotmail.com

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#1338441 - 04/15/13 05:11 PM Re: Having a Necropsy/Histopath done the PROPER way!! [Re: Srlb]
GliderNursery Offline
Glideritis Anonymous

Registered: 09/14/07
Posts: 20049
Loc: North Central Ohio
A local owner had a situation where her glider passed and it was highly recommended by the vet to have a local vet do the gross necropsy and to preserve the organs and all tissues needed. Doing so greatly increases the chances of more accurate necropsy reports and reduces the increased degradation of tissue/organs as a result of natural decomposition. Even a few days can alter the results.
_________________________
Shelly

Don't sacrifice quality information for convenient information.


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