I will have had my glider girls a week tomorrow. Today I noticed that one of my girls used both hands to pull open her pouch and started licking inside of it. Could there be a Joey in there?

All female gliders will do that to clean their pouch. Unless your female has been exposed to an intact male there is no reason to believe that she is carrying joeys.

No idea if she was exposed or not I have only had them a week. I got them from The Pet Glider. I was supposed to get a different pair but they found that one of them had Joey's in her pouch. So now I am wondering if she could have come to me very pregnant or newly having a Joey in pouch.

Take a deep breath and relax. If she starts showing bumps she has joeys in pouch.

If not know worries.

How old is she? Is she a retired breeder?

She is 7 and yes she is a retired breeder. I'm not worried or stressing about it just curious about the possibilities

Just keep her in with her buddy and watch her.

If she does have a joey or joeys hopefully the other female won't pull them, but help raise them. If she is carrying then you would neuter any male joeys and you would have a nice little colony.